Journal: Molecular Medicine Reports

Article Title: Sonic hedgehog protein regulates fibroblast growth factor 8 expression in metanephric explant culture from BALB/c mice: Possible mechanisms associated with renal morphogenesis

doi: 10.3892/mmr.2016.5614

Figure Lengend Snippet: Primer sequences used for polymerase chain reaction.

Article Snippet: Subsequently, sections were incubated overnight at 4°C with polyclonal rabbit anti-Fgf8 (dilution, 1:100; cat. no. ABIN1107218; Bioss Inc., Woburn, MA, USA) and polyclonal rabbit anti-Fgf10 (dilution, 1:100; cat. no. ABIN392510; Bioss Inc.) primary antibodies.

Techniques:

Journal: Molecular Medicine Reports

Article Title: Sonic hedgehog protein regulates fibroblast growth factor 8 expression in metanephric explant culture from BALB/c mice: Possible mechanisms associated with renal morphogenesis

doi: 10.3892/mmr.2016.5614

Figure Lengend Snippet: mRNA expression levels of (A) SHH, (B) Fgf8 and (C) Fgf10 in normal BALB/c mouse embryonic kidney tissue explants at E11.5, E12.5, E13.5 and E14.5 gestational ages. For each sample, the mRNA expression levels were normalized to GAPDH mRNA expression (n=7 for each treatment group ). * P<0.05, **P<0.01 and *** P<0.001 vs. E11.5 group. SHH, sonic hedgehog; Fgf, fibroblast growth factor; E, embryonic day.

Article Snippet: Subsequently, sections were incubated overnight at 4°C with polyclonal rabbit anti-Fgf8 (dilution, 1:100; cat. no. ABIN1107218; Bioss Inc., Woburn, MA, USA) and polyclonal rabbit anti-Fgf10 (dilution, 1:100; cat. no. ABIN392510; Bioss Inc.) primary antibodies.

Techniques: Expressing

Journal: Molecular Medicine Reports

Article Title: Sonic hedgehog protein regulates fibroblast growth factor 8 expression in metanephric explant culture from BALB/c mice: Possible mechanisms associated with renal morphogenesis

doi: 10.3892/mmr.2016.5614

Figure Lengend Snippet: mRNA expression levels of (A) Fgf8 and (B) Fgf10 mRNA levels in BALB/c mouse kidney tissue explant cultures following treatment with 1% BSA, 1% BSA + SHH and 1% BSA + SHH + cyclopamine for 4 days. Fgf8 and Fgf10 expression levels were normalized to GAPDH mRNA expression levels (n=7 for each treatment group). ** P<0.01 vs. 1% BSA-alone group; ## P<0.01 vs. 1% BSA+SHH group. Fgf, fibroblast growth factor; BSA, bovine serum albumin; SHH, sonic hedgehog.

Article Snippet: Subsequently, sections were incubated overnight at 4°C with polyclonal rabbit anti-Fgf8 (dilution, 1:100; cat. no. ABIN1107218; Bioss Inc., Woburn, MA, USA) and polyclonal rabbit anti-Fgf10 (dilution, 1:100; cat. no. ABIN392510; Bioss Inc.) primary antibodies.

Techniques: Expressing

Journal: Molecular Medicine Reports

Article Title: Sonic hedgehog protein regulates fibroblast growth factor 8 expression in metanephric explant culture from BALB/c mice: Possible mechanisms associated with renal morphogenesis

doi: 10.3892/mmr.2016.5614

Figure Lengend Snippet: Analysis of Fgf8 and Fgf10 expression in BALB/c mouse kidney tissue explant cultures following treatment with 1% BSA (control) or 1% BSA + SHH for 4 days. (A) Immunohistochemical analysis and (B) integral optical densities of Fgf8 and Fgf10 expression levels in the different treatment groups. (C) Western blot analysis and (D) quantification of western blot results, showing the protein expression levels of Fgf8 and Fgf10 expression levels in the various groups. The protein expression levels were normalized to β-actin (n=7 for each treatment group). Scale bar=125 µ m. * P<0.05 and ** P<0.01 vs. 1% BSA-alone group; # P<0.05 and ## P<0.01 vs. 1% BSA + SHH group. Fgf, fibroblast growth factor; BSA, bovine serum albumin; SHH, sonic hedgehog.

Article Snippet: Subsequently, sections were incubated overnight at 4°C with polyclonal rabbit anti-Fgf8 (dilution, 1:100; cat. no. ABIN1107218; Bioss Inc., Woburn, MA, USA) and polyclonal rabbit anti-Fgf10 (dilution, 1:100; cat. no. ABIN392510; Bioss Inc.) primary antibodies.

Techniques: Expressing, Immunohistochemical staining, Western Blot

Journal: British Journal of Cancer

Article Title: Fibroblast growth factor 8 is expressed at higher levels in lactating human breast and in breast cancer

doi: 10.1038/sj.bjc.6600213

Figure Lengend Snippet: Western blots showing the specificity of the anti-FGF8 antibody. One hundred ng of FGF1 (lane 1), FGF2 (lane 2), FGF7 (lane 3), FGF9 (lane 4) and FGF8 (lane 5) were run on a 15% polyacrylamide gel and transferred onto nitrocellulose filters. These were probed with anti-FGF8 monoclonal antibody.

Article Snippet: The monoclonal anti-FGF8 antibody used in this study (R&D Systems) was produced from a mouse hybridoma in which the immunogen was an E. coli -derived recombinant mouse fibroblast growth factor 8b.

Techniques: Western Blot

Journal: British Journal of Cancer

Article Title: Fibroblast growth factor 8 is expressed at higher levels in lactating human breast and in breast cancer

doi: 10.1038/sj.bjc.6600213

Figure Lengend Snippet: Expression of FGF8 in human breast cancer tissue. Paraffin sections of invasive ductal carcinoma ( A , B ), invasive lobular carcinoma ( C , D ) and ductal carcinoma in situ (DCIS) ( E , F ), were analysed by immunohistochemistry using an antibody against FGF-8 ( A , C , E ) or the equivalent concentration of non-immune mouse IgG ( B , F ). ( D ) Shows absence of anti-FGF8 staining after pre-incubation with 100-fold excess of recombinant FGF8. (Original magnification A – D , ×400; E , F , ×200).

Article Snippet: The monoclonal anti-FGF8 antibody used in this study (R&D Systems) was produced from a mouse hybridoma in which the immunogen was an E. coli -derived recombinant mouse fibroblast growth factor 8b.

Techniques: Expressing, In Situ, Immunohistochemistry, Concentration Assay, Staining, Incubation, Recombinant

Journal: British Journal of Cancer

Article Title: Fibroblast growth factor 8 is expressed at higher levels in lactating human breast and in breast cancer

doi: 10.1038/sj.bjc.6600213

Figure Lengend Snippet: Expression of FGF8 in non-malignant human breast tissue. Paraffin sections of normal breast tissue from reduction mammoplasty tissue ( A , B ), fibroadenoma ( C , D ) and paraffin sections containing elements of both invasive ductal carcinoma and adjacent normal breast tissues ( E , F ), were analysed by immunohistochemistry using an antibody against FGF8 ( A , C , E , F ) or the equivalent concentration of non-immune mouse IgG ( B , D ). (Original magnification A – B , ×400; C – F ×200).

Article Snippet: The monoclonal anti-FGF8 antibody used in this study (R&D Systems) was produced from a mouse hybridoma in which the immunogen was an E. coli -derived recombinant mouse fibroblast growth factor 8b.

Techniques: Expressing, Immunohistochemistry, Concentration Assay

Journal: British Journal of Cancer

Article Title: Fibroblast growth factor 8 is expressed at higher levels in lactating human breast and in breast cancer

doi: 10.1038/sj.bjc.6600213

Figure Lengend Snippet: Histograms showing the level of FGF8 immunoreactivity in malignant and non-malignant breast tissue. Tissues were scored as +, ++ or +++ for FGF8 staining. ( A ) Shows percentages of patients with each level of staining in: 1=Fibroadenoma; 2=Normal breast tissue (reducton mammoplasty); 3=Lactating breast tissue. ( B ) Shows FGF8 staining patterns in Invasive Ductal Carcinoma Grade 1, 2 and 3; DCIS and Lobular carcinoma.

Article Snippet: The monoclonal anti-FGF8 antibody used in this study (R&D Systems) was produced from a mouse hybridoma in which the immunogen was an E. coli -derived recombinant mouse fibroblast growth factor 8b.

Techniques: Staining

Journal: British Journal of Cancer

Article Title: Fibroblast growth factor 8 is expressed at higher levels in lactating human breast and in breast cancer

doi: 10.1038/sj.bjc.6600213

Figure Lengend Snippet: FGF8 staining in lactating mammary gland. Paraffin sections of lactating mammary gland tissue were stained with an antibody against FGF8 ( A , C ) or with the equivalent concentration of non-immune mouse IgG ( B , D ). (Original magnification A , B , ×200; C , D , ×400).

Article Snippet: The monoclonal anti-FGF8 antibody used in this study (R&D Systems) was produced from a mouse hybridoma in which the immunogen was an E. coli -derived recombinant mouse fibroblast growth factor 8b.

Techniques: Staining, Concentration Assay

Journal: British Journal of Cancer

Article Title: Fibroblast growth factor 8 is expressed at higher levels in lactating human breast and in breast cancer

doi: 10.1038/sj.bjc.6600213

Figure Lengend Snippet: Western blots demonstrating the presence of FGF8 in human breast milk. Proteins from human milk samples were run on a 12% polyacrylamide gel, transferred onto nitrocellulose and probed using the monoclonal antibody against FGF8. Lanes A – E contain milk proteins and lane F contains 100 ng of recombinant FGF8.

Article Snippet: The monoclonal anti-FGF8 antibody used in this study (R&D Systems) was produced from a mouse hybridoma in which the immunogen was an E. coli -derived recombinant mouse fibroblast growth factor 8b.

Techniques: Western Blot, Recombinant

Journal: Cell Stem Cell

Article Title: Competition for Mitogens Regulates Spermatogenic Stem Cell Homeostasis in an Open Niche

doi: 10.1016/j.stem.2018.11.013

Figure Lengend Snippet:

Article Snippet: Mouse monoclonal anti-FGF8 (used at 1:1000) , Kyowa Hakko Kirin , Cat#KM1334, Lot KM1334-2.

Techniques: Recombinant, Imaging, Staining, Labeling, RNAscope, Multiplex Assay, Transfection, Reporter Assay, Microarray, Clone Assay

Journal: Stem Cell Research & Therapy

Article Title: Generation of tooth–periodontium complex structures using high-odontogenic potential dental epithelium derived from mouse embryonic stem cells

doi: 10.1186/s13287-017-0583-5

Figure Lengend Snippet: mES cell-derived epithelium. a Cell morphology. mES cells present a colony growth character, each clone with an obvious boundary. EBs are round balls. One day after attachment, cells began to climb out peripheral from the EBs (radial growth). A large number of epithelioid cells presented on day 3 and later. b qRT-PCR showed representative downregulation of mES cell-specific transcription factor (Oct4) and the expression of keratin epithelial marker k14 was upregulated on day 3. Expression of early ectoderm marker P63 and K18 was relative lower on day 3. FGF8 expression was up regulated on day 3 while BMP4 showed a high expression level. c Immunostaining of the day 3 cells for FGF8 and BMP4. BMP4 bone morphogenic protein 4, D day, EB embryoid body, FGF8 fibroblast growth factor 8, mESC mouse embryonic stem cell

Article Snippet: Antibodies against the following proteins were used at the indicated dilutions: paired box gene 9 (PAX9; rabbit, 1:200; Santa Cruz Biotechnology, Heidelberg, Germany), PITX2 (mouse, 1:200; Santa Cruz Biotechnology), FGF8 (mouse, 1:200; Santa Cruz Biotechnology), BMP4 (mouse, 1:200; Santa Cruz Biotechnology), and AMBN (mouse, 1:200; Santa Cruz Biotechnology).

Techniques: Derivative Assay, Quantitative RT-PCR, Expressing, Marker, Immunostaining

Journal: Analytical Cellular Pathology (Amsterdam)

Article Title: Evaluation and Immunolocalization of BMP4 and FGF8 in Odontogenic Cyst and Tumors

doi: 10.1155/2018/1204549

Figure Lengend Snippet: Evaluation and immunohistochemical score of stages of odontogenesis.

Article Snippet: The antibody clone used for detection of FGF8 was PA1216 (Boster, Pleasanton, CA, polyclonal antibody).

Techniques: Immunohistochemical staining

Journal: Analytical Cellular Pathology (Amsterdam)

Article Title: Evaluation and Immunolocalization of BMP4 and FGF8 in Odontogenic Cyst and Tumors

doi: 10.1155/2018/1204549

Figure Lengend Snippet: Evaluation and immunohistochemical score of odontogenic tumors and odontogenic cyst.

Article Snippet: The antibody clone used for detection of FGF8 was PA1216 (Boster, Pleasanton, CA, polyclonal antibody).

Techniques: Immunohistochemical staining

Journal: Analytical Cellular Pathology (Amsterdam)

Article Title: Evaluation and Immunolocalization of BMP4 and FGF8 in Odontogenic Cyst and Tumors

doi: 10.1155/2018/1204549

Figure Lengend Snippet: Intergroup comparison of expression of BMP4 and FGF8 in odontogenic tumors and odontogenic cyst.

Article Snippet: The antibody clone used for detection of FGF8 was PA1216 (Boster, Pleasanton, CA, polyclonal antibody).

Techniques: Comparison, Expressing